CCL5 Production in the Breast Tumor Microenvironment
Current literature suggests that Chemokine (C-C motif) ligand 5 (also known as CCL5) is an important cytokine for communication between within the breast tumor microenvironment, and is related to greater rates of breast tumor metastases. In addition, previous experiments in our lab show increased levels of CCL5 in the conditioned media of co-cultures of adipose stromal cells and breast tumor cells.
This project aims to determine the cellular source of CCL5 within the breast tumor environment by treating adipose stromal cells and breast tumor cells with the conditioned media of the opposite cell type. In addition to analyzing secretion of CCL5 protein by each cell type, we are measuring intracellular CCL5 mRNA levels using qRT-PCR.
CCL5 protein concentrations in the conditioned media of (A) adipose-derived stem cells and (B) adipose-derived preadipocytes cultured with and without the presence of breast tumor cells. CCL5
Daniel Berrebi, an undergraduate researcher, analyzing CCL5 concentrations of conditioned cell culture media.