Modeling of ALL and the Bone Marrow Microenvironment
Chemoresistant minimal residual disease (MRD) that persists following cessation of therapy contributes to aggressive relapse of acute lymphoblastic leukemia and is supported through interacts with the bone marrow microenvironment. We have developed an in-vitro co-culture method that models these interactions to investigate the roll of the bone marrow microenvironment in the promotion of chemotherapy resist ALL cells.
Bone marrow microenvironment influence on ALL cell metabolism
Cancer cell metabolism is known to impact the proliferation and survival of tumor cells. Our laboratory, focuses on changes in ALL cell metabolism that are brought on through interactions with the bone marrow microenvironment.
Bone Marrow Function Following Chemotherapy
A critical element of patient outcome following bone marrow transplantation is efficient recovery of hematopoiesis. We have a long standing interest in understanding the influence of aggressive chemotherapy, often used clinically prior to transplant, on a patient's bone marrow capacity to support this recovery.
Bone marrow microenvironment influence on leukemic miRNA expression
MiRNAs define one regulatory class of small non-coding RNAs that have been shown to be increasingly important in hematopoietic cell biology. Alteration of miRNA expression has been shown to impact on tumor cell survival and therapeutic response. These observations provide the rationale for considering regulation of tumor cell miRNA expression profiles, or miRNA function, by bone marrow microenvironment signals as one mechanism by which the marrow niche influences leukemic cell characteristics.
Bone Marrow Microenvironment influence over Leukemia Quiescence and Chemotherapy Resistance through Modulation of BCL6
Expression of BCL6 has been shown to be an important protein in leukemic cells that promotes chemotherapy resistance. Our laboratory looks at how the bone marrow microenvironment influences that expression of BCL6 in ALL cells and how this impact their resistance to chemotherapy.