In Vivo Imaging of Oral Cancer Progression

“In Vivo Imaging of Oral Cancer Progression ”

Visualization of the initial steps of HNSCC invasion will greatly aid in understanding the shared and unique steps this particular tumor type undertakes during the metastatic cascade. HNSCC typically undergoes collective invasion, where cell-cell attachment is largely preserved and epithelial-to-mesenchymal transition (EMT) is minimal. While EMT-based invasion has been extensively studied, the elements that govern collective invasion in HNSCC and other tumor types are emerging. We have adapted the use of multiple imaging modalities to allow for in vivo imaging of invasion and metastasis in mice harboring HNSCC (see Project 2). These methods include the use of 2-photon microscopic imaging of fluorescently-labeled tumors in mouse tongues, bioluminescent imaging of tumor growth and regional metastasis using IVIS imaging and high frequency ultrasound imaging of cervical lymph nodes to monitor tumor-induced vascular changes and nodal metastasis. This work extensively utilizes the Animal Models and Imaging Facility

In vivo imaging of HNSCC in live mice. A. Digital rendering of a non-viral oral squamous cell papilloma on the tip of a live mouse tongue. The mouse was anesthetized and the tongue imaged by high frequency ultrasound using the Vevo 2100 imaging platform. Rendering of the papilloma (cyan) and tongue tip (purple) were conducted using Vevo software. B. In situ imaging of an orthotopic HNSCC tumor in mouse tongue. OSC19 HNSCC cells stably expressing LifeAct-mCherry as a fluorescent marker were injected into the tongue and allowed to form a tumor over four weeks. The tongue was resected and imaged by 2-photon microscopy, with z-stack sections deconvoluted, reconstructed and rendered using Nikon NIS-Elements software. The primary tumor is evident, along with multiple invasive cellular groups denoted by arrowheads. Bar = 100µm.

Figure and legend for (B )was adapted from http://www.jove.com/video/2941/multi-photon-imaging-tumor-cell-invasion-an-orthotopic-mouse-model