Publications of Scott A Weed, PhD.

NEDD9 regulates actin dynamics through cortactin deacetylation in an AURKA/HDAC6-dependent manner. Kozyreva VK, McLaughlin SL, Livengood RH, Calkins RA, Kelley LC, Rajulapati A, Ice RJ, Smolkin MB, Weed SA, Pugacheva EN. Mol Cancer Res. 2014;12(5):681-693 PMC4020952.

NEDD9 depletion leads to MMP14 inactivation by TIMP2 and prevents invasion and metastasis. McLaughlin SL, Ice RJ, Rajulapati A, Kozyulina PY, Livengood RH, Kozyreva VK, Loskutov YV, Culp M, Weed SA, Ivanov AV, Pugacheva EN. Mol Cancer Res. 2014;12(1):69-81. PMC3946989.

Ableson kinases negatively regulate invadopodia function and invasion in head and neck squamous cell carcinoma by inhibiting an HB-EGF autocrine loop. Hayes KE, Walk EL, Ammer AG, Kelley LC, Martin KH, Weed SA. Oncogene. 2013;32(40):4766-4777. PMC3896120.

Phosphorylation of the alternative mRNA splicing factor 45 (SPF45) by Clk1 regulates its splice site utilization, cell migration and invasion. Liu Y, Conaway L, Rutherford BJ, Al-Ayoubi AM, Thompson BA, Zheng H, Weed SA, Eblen ST. Nucleic Acids Res. 2013;41(9):4949-4962. PMC3643583.

Metastatic MTLn3 and non-metastatic MTC adenocarcinoma cells can be differentiated by Pseudomonas aeruginosa. Novotny MJ, Bridge DR, Martin KH, Weed SA, Wysolmerski RB, Olson JC. Biol Open. 2013;2(9):891-900. PMC3773335.

SRChing for the substrates of Src. Reynolds AB, Kanner SB, Bouton AH, Schaller MD, Weed SA, Flynn DC, Parsons JT. Oncogene. 2013; [Epub ahead of print]

Src binds cortactin through a SH2 domain cystine-mediated linkage. Evans JV, Ammer AG, Jett JE, Bolcato CA, Breaux JC, Martin KH, Culp M, Gannett PM, Weed SA. J Cell Sci. 2012;125(Pt 24):6185-6197. PMC3585525.

Cortactin Is a substrate of activated Cdc42-Associated Kinase 1 (ACK1) during ligand-induced epidermal growth factor receptor downregulation. Kelley LC, Weed SA. PLoS One. 2012;7(8):e44363 PMC3431376.

Quantitative measurement of invadopodia-mediated extracellular matrix proteolysis in single and multicellular contexts. Martin KH, Hayes KE, Walk EL, Ammer AG, Markwell SM, Weed SA. J Vis Exp. 2012;(66):4419 PMC3606055.

Effects of tobacco smoking and nicotine on cancer treatment. Petros WP, Younis IR, Ford JN, Weed SA. Pharmacotherapy. 2012;32(10):920-931. PMC3499669.

Multi-photon imaging of tumor cell invasion in an orthotopic mouse model of oral squamous cell carcinoma. Ammer AG, Hayes KE, Martin KH, Zhang L, Spirou GA, Weed SA. J Vis Exp. 2011;(53) PMC3196194.

Further insights into cortactin conformational regulation. Evans JV, Kelley LC, Hayes KE, Ammer AG, Martin KH, Weed SA. Bioarchitecture. 2011;1(1):21-23. PMC3158636.

Revisiting the ERK/Src cortactin switch. Kelley LC, Hayes KE, Ammer AG, Martin KH, Weed SA. Commun Integr Biol. 2011;4(2):205-207. PMC3104580.

Recently identified biomarkers that promote lymph node metastasis in head and neck squamous cell carcinoma. Walk EL, Weed SA. Cancers. 2011;3(1):747-772.

Oncogenic Src requires a wild-type counterpart to regulate invadopodia maturation. Kelley LC, Ammer AG, Hayes KE, Martin KH, Machida K, Jia L, Mayer BJ, Weed SA. J Cell Sci. 2010;123(pt22):3923-3932. PMC2972274.

Cortactin phosphorylated by ERK1/2 localizes to sites of dynamic actin regulation and is required for carcinoma lamellipodia persistence. Kelley LC, Hayes KE, Ammer AG, Martin KH, Weed SA. PLoS One. 2010;5(11):e1384 PMC2973953.

Cytoskeleton alterations in melanoma: aberrant expression of cortactin, an actin-binding adapter protein, correlates with melanocytic tumor progression. Xu XZ, Garcia MV, Li TY, Khor LY, Gajapathy RS, Spittle C, Weed SA, Lessin SR, Wu H. Mod Pathol. 2010;23(2):187-196. PMC2827925.

Saracatinib impairs head and neck squamous cell carcinoma invasion by disrupting invadopodia function. Ammer AG, Kelley LC, Hayes KE, Evans JV, Lopez-Skinner LA, Martin KH, Frederick B, Rothschild BL, Raben D, Elvin P, Green TP, Weed SA. J Cancer Sci Ther. 2009;1(2):52-61. PMC2875552.

Cortactin is a functional target of E-cadherin-activated Src family kinases in MCF7 epithelial monolayers. Ren G, Helwani FM, Verma S, McLachlan RW, Weed SA, Yap AS.J Biol Chem. 2009;284(28):18913-18922. PMC2707247.

Cortactin branches out: roles in regulating protrusive actin dynamics. Ammer AG, Weed SA. Cell Motil Cytoskeleton. 2008;65(9):687-707. PMC2561250.

Phosphorylation of AFAP-110 affects podosome lifespan in A7r5 cells. Dorfleutner A, Cho YJ, Vincent DM, Cunnick JM, Lin H, Weed SA, Stehlik C, Flynn DC. J Cell Sci. 2008;121(Pt 14):2394-2405.

Actin cytoskeletal mediators of motility and invasion amplified and overexpressed in head and neck cancer. Kelley LC, Shahab S, Weed SA. Clin Exp Metastasis. 2008;25(4):289-304.

Overexpression of cyclin D1 and cortactin is primarily independent of gene amplification in salivary gland adenoid cystic carcinoma. Greer RO, Jr., Said S, Shroyer KR, Marileila VG, Weed SA. Oral Oncol. 2007;43(8):735-741.

Src phosphorylation of cortactin enhances actin assembly.Tehrani S, Tomasevic N, Weed SA, Sakowicz R, Cooper JA.Proc Natl Acad Sci USA. 2007;104(29):11933-11938. PMC1924558.

PI3K activation is required for PMA-directed activation of cSrc by AFAP-110.Walker VG, Ammer AG, Cao Z, Clump AC, Jiang BH, Kelley LC, Weed SA, Zot HM, Flynn DC.Am J Physiol Cell Physiol. 2007;293(1):C119-C132.

Cortactin overexpression regulates actin-related protein 2/3 complex activity, motility, and invasion in carcinomas with chromosome 11q13 amplification.Rothschild BL, Shim AH, Ammer AG, Kelley LC, Irby KB, Head JA, Chen L, Varella-Garcia M, Sacks PG, Frederick B, Raben D, Weed SA. Cancer Res. 2006;66(16):8017-8025.

Rac2D57N, a dominant inhibitory Rac2 mutant that inhibits p38 kinase signaling and prevents surface ruffling in bone-marrow-derived macrophages. Abell AN, DeCathelineau AM, Weed SA, Ambruso DR, Riches DW, Johnson GL. J Cell Sci. 2004;117(Pt 2):243-255.

Cortactin and Crk cooperate to trigger actin polymerization during Shigella invasion of epithelial cells. Bougneres L, Girardin SE, Weed SA, Karginov AV, Olivo-Marin JC, Parsons JT, Sansonetti PJ, Van Nhieu GT. J Cell Biol. 2004;166(2):225-235. PMC2172305.

Actin depolymerization-induced tyrosine phosphorylation of cortactin: the role of Fer kinase. Fan L, Di Ciano-Oliveira C, Weed SA, Craig AW, Greer PA, Rotstein OD, Kapus A. Biochem J. 2004;380(Pt 2):581-591. PMC1224196.

Protein kinase Calpha activates c-Src and induces podosome formation via AFAP-110. Gatesman AS, Walker VG, Baisden JM, Weed SA, Flynn DC. Mol Cell Biol. 2004;24(17):7578-7597.PMC506973.

Cortactin is necessary for E-cadherin-mediated contact formation and actin reorganization. Helwani FM, Kovacs EM, Paterson AD, Verma S, Ali RG, Fanning AS, Weed SA, Yap AS. J Cell Biol. 2004;164(6):899-910. PMC2172285.

Characterization of an ankyrin repeat-containing Shank2 isoform (Shank2E) in liver epithelial cells. McWilliams RR, Gidey E, Fouassier L, Weed SA, Doctor RB.
Biochem J. 2004;380(Pt 1):181-191.
PMC1224161.

MEKK1 regulates calpain-dependent proteolysis of focal adhesion proteins for rear-end detachment of migrating fibroblasts. Cuevas BD, Abell AN, Witowsky JA, Yujiri T, Johnson NL, Kesavan K, Ware M, Jones PL, Weed SA, DeBiasi RL, Oka Y, Tyler KL, Johnson GL. EMBO J. 2003;22(13):3346-3355. PMC165646.

Cortactin tyrosine phosphorylation requires Rac1 activity and association with the cortical actin cytoskeleton. Head JA, Jiang D, Li M, Zorn LJ, Schaefer EM, Parsons JT, Weed SA. Mol Biol Cell. 2003;14(8):3216-3229. PMC181562.

Cortactin interacts with WIP in regulating Arp2/3 activation and membrane protrusion. Kinley AW, Weed SA, Weaver AM, Karginov AV, Bissonette E, Cooper JA, Parsons JT. Curr Biol. 2003;13(5):384-393.

Dynamin2 and cortactin regulate actin assembly and filament organization. Schafer DA, Weed SA, Binns D, Karginov AV, Parsons JT, Cooper JA. Curr Biol. 2002;12(21):1852-1857.

Cortactin promotes and stabilizes Arp2/3-induced actin filament network formation. Weaver AM, Karginov AV, Kinley AW, Weed SA, Li Y, Parsons JT, Cooper JA. Curr Biol. 2001;11(5):370-374.

Cortactin: coupling membrane dynamics to cortical actin assembly. Weed SA, Parsons JT. Oncogene. 2001;20(44):6418-6434.

Dynamic molecular modeling of pathogenic mutations in the spectrin self-association domain. Zhang Z, Weed SA, Gallagher PG, Morrow JS. Blood. 2001;98(6):1645-1653.

Regulation of c-SRC activity and function by the adapter protein CAS. Burnham MR, Bruce-Staskal PJ, Harte MT, Weidow CL, Ma A, Weed SA, Bouton AH. Mol Cell Biol. 2000;20(16):5865-5878. PMC86064.

Focal adhesion kinase: a regulator of focal adhesion dynamics and cell movement. Parsons JT, Martin KH, Slack JK, Taylor JM, Weed SA. Oncogene. 2000;19(49):5606-5613.

Cortactin localization to sites of actin assembly in lamellipodia requires interactions with F-actin and the Arp2/3 complex. Weed SA, Karginov AV, Schafer DA, Weaver AM, Kinley AW, Cooper JA, Parsons JT. J Cell Biol. 2000;151(1):29-40. PMC2189811.

Identification of a novel cortactin SH3 domain-binding protein and its localization to growth cones of cultured neurons. Du Y, Weed SA, Xiong WC, Marshall TD, Parsons JT. Mol Cell Biol. 1998;18(10):5838-5851. PMC109170.

Translocation of cortactin to the cell periphery is mediated by the small GTPase Rac1. Weed SA, Du Y, Parsons JT. J Cell Sci. 1998;111 ( Pt 16):2433-2443.

Mutation of a highly conserved residue of betaI spectrin associated with fatal and near-fatal neonatal hemolytic anemia. Gallagher PG, Petruzzi MJ, Weed SA, Zhang Z, Marchesi SL, Mohandas N, Morrow JS, Forget BG. J Clin Invest. 1997;99(2):267-277. PMC507794.

The lethal hemolytic mutation in beta I sigma 2 spectrin Providence yields a null phenotype in neonatal skeletal muscle. Weed SA, Stabach PR, Oyer CE, Gallagher PG, Morrow JS. Lab Invest. 1996;74(6):1117-1129.

Recurrent fatal hydrops fetalis associated with a nucleotide substitution in the erythrocyte beta-spectrin gene. Gallagher PG, Weed SA, Tse WW, Benoit L, Morrow JS, Marchesi SL, Mohandas N, Forget BG. J Clin Invest. 1995;95(3):1174-1182. PMC441455.

A partial structural repeat forms the heterodimer self-association site of all beta-spectrins. Kennedy SP, Weed SA, Forget BG, Morrow JS. J Biol Chem. 1994;269(15):11400-11408.

Beta II-spectrin (fodrin) and beta I epsilon 2-spectrin (muscle) contain NH2- and COOH-terminal membrane association domains (MAD1 and MAD2). Lombardo CR, Weed SA, Kennedy SP, Forget BG, Morrow JS. J Biol Chem. 1994;269(46):29212-29219.