IVIS®Lumina Imaging System

The equipment that will be used for in vivo imaging is the IVIS® Lumina Imaging System. The components of this system include a cooled CCD camera, Imaging Chamber, acquisition computer with Living Image® software, and Integrated Gas Anesthesia system.

CCD Camera:

  • 13 x 13 mm square with 1024 x 1024 pixels 13 microns in width
  • Back-thinned, back illuminated grade 1 CCD
  • 16 bit digitizer
  • Thermoelectrically (Peltier) cooled to -90°C

Imaging Chamber:

  • Light-tight
  • High light collection lens, f/0.95 – f/16
  • 8 position emission filter wheel
  • LED lamps for photographic images
  • Heated stage to maintain optimum body temperature
  • Motor controlled stage, filter wheel, lens position, and f-stop

Integrated Gas Anesthesia:

  • Gas anesthesia (isoflurane) ports and 5 position manifold within imaging chamber

Living Image® Software:

  • Photo brightness and color bar adjustment
  • Image annotation tools
  • Region of interest (ROI) measurements
  • Absolute calibration of image intensity against NIST radiance standards
  • Corrections for cosmic rays, dark charge, and flat field
  • Printing, layout, and image export functions

IVIS System Control Panel:

  • Controls for both fluorescence and bioluminescence imaging
  • Manual and auto focus
  • Setting of exposure time, binning, f/stop, and field view
  • Fluorescence lamp and filter controls
  • Acquisition setup of a single image or an image sequence

Metastasis Models

Orthotopic injections of luciferase positive oral cancer cells. In addition to the primary oral tumor, metastasis to the lymph node is visible in the mouse on the bottom while the mouse on the top has no evidence of metastasis.

Intravenous (tail vein) injections of luciferase positive breast cancer cells. This model allows investigators to study metastatic properties of tumor cells. In this study we see the cells have homed to the lungs where they have colonized and formed tumors.

Bioluminescent Bacteria

Caliper Life Sciences has engineered light producing bacteria. This can be used in mouse models to study infection and biofilms. This image is a blood agar plate that has been streaked with bioluminescent Staphylococcus aureus.