Nikon A1R Confocal

Demonstration of Nikon A1R Confocal microscope with SIMThe Nikon A1R Confocal with N-SIM provides live cell confocal imaging in four colors, super-resolution Structured Illumination Microscopy (SIM), and epifluorescent and brightfield imaging.

Microscope Specs

• Nikon Eclipse Ti microscope
• Tokai Hit Stage Top incubator (Temperature, Humidity, and CO2 control)

A1R Confocal Specs

• Lasers: 405, 488, 561, and 640
• Galvano and Resonant scanners

Widefield Microscopy Specs

• Hamamatsu Orca Flash 4.0 monochrome cMOS camera
• X-Cite Excelitas LED Fluorescent Light Source
• Fluorescent Filter sets for: DAPI, FITC, and TRITC

SIM Specs

• Hamamatsu Orca Flash 4.0 monochrome cMOS camera
• Lasers: 488, 561, and 640

Objectives:

• Plan Apo  10X / 0.5 WD: 4 mm
• Plan Apo VC 20X DIC N2 / 0.8 WD: 1mm
• Plan Fluor 40X Oil DIC H N2 / 1.3 WD: 240 µm
• Plan Apo  60X Oil / 1.4 WD: 130 µm
• SR Apo TIRF 100X Oil / 1.5 WD: 120 µm

Protocols:

Nikon A1R Confocal User Guide
Nikon Widefield and Epifluorescence User Guide
Nikon A1R – Setting Kohler