NLOM

Upright microscope provides multiphoton imaging in 2 channels –green and red.

 Microscope Specs

  • Upright microscope system (MOveable Microscope, Sutter Instruments)
  • 3 -axis motorized control of the objective and a galvanometric scanner
  • 2 PMTS for imaging in green and red
  • Ti:sapphire tunable laser, pumped by a Verdi laser
  • optical parametric oscillator (OPO)
  • isoflurane anesthesia system for intravital imaging
  • in vitro pressure-based tissue perfusion system

 

Objectives:

  • 4X U-Plan FL / 0.13             Air
  • 10X U-Plan FL / 0.3             Air
  • 10X UM Plan FL-N /0.3 Water
  • 20X LC Plan FL / 0.4             Air
  • 20X U-Plan Apo/ 0.75 Air
  • 20X Plan Apo / 1.0 Water
  • 40X LC Plan FL / 0.6             Air
  • 40X LUMPlan FL-IR/ 8       Water
  • 60X LUMPlan FL-IR / 0.9 Water
  • 60X U Plan S Apo / 1.35 Oil
  • 60X U Plan S Apo-IR / 1.2 Water
  • 60X LC Plan FL / 0.7             Air
  • 100X Plan Apo / 1.4             Oil

 

Protocols:

  • NLOM Start up and Shut down
  • NLOM User Guide
  • Tissue Perfusion Set up