Zeiss Violet Confocal

Demonstration images of Zeiss Violet confocal.Upright confocal microscope provides confocal imaging in four channels -- blue, green, red, and far red.

Microscope Specs

  • Zeiss Axiolmager Z1 microscope
  • Zeiss LSM 510 Confocal (v4.2 SP1)                                                                                                                                                                                                                                                                                                                                    
Type Magnification Numerical
Aperture
Immersion Contrast Working
Distance
EC Plan-Neofluar 10x 0.30 Air   5.2 mm
Plan-Apochromat 20x 0.80 Air   0.55 mm
LD Plan-Neofluar 20x 0.40 Air   8.4 mm
EC Plan-Neofluar 40x 1.30 Oil DIC

0.21 mm

LD Plan-Neofluar

40x 0.60 Air   2.9
W N-Achroplan 40x 0.75 W   2.1 mm
Plan-Apochromat 63x 1.40 Oil DIC 0.19 mm
LD Plan-Neoflaur 63x 0.75     2.2 mm

  Filter Sets for Visualizing by Eye  

  Order # Excitation Beam Splitter Emission
DAPI (Filter Set 02) 488002-9901-000 G 365 FT 395 LP 420
FITC (Filter Set 09) 488009-9901-000 BP 450-490 FT 510 LP 515
RHOD (Filter Set 15) 488015-0000-000 BP 546/12 FT 580 LP 590

  Lasers

Argon/2 458,477,488,514 nm 30.0 mW
HeNe 543 543 nm 1.2 mw
HeNe 633 633 nm 5.0 mW
Diode 405-30 405 nm 30.0 mW

    Emission Filters for Visualizing by Confocal

Ch1 Ch2 Ch3
LP 420 LP 420 LP 505
LP 475 LP 475 LP 560
LP 505 LP 505 LP 615 (QD 605)
LP 520 BP 420-480 LP 650 (QD 655)
LP 560 BP 465-510 IR BP 505-530 (QD 525)
LP 615 BP 505-530 BP 505-550
LP 650 BP 505-575 IR BP 520-555 IR (QD 545)
    BP 560-615 IR (QD 565)

Objectives
Spectral Viewer
Fluorophore Chart

Protocols