Equipment

The WVU PDXCF is located in Room V703 of the Robert C. Byrd Health Sciences Center (HSC) and encompasses approximately 1605 sq. ft. of new laboratory and animal housing space on the Health Sciences Center Campus of West Virginia University (WVU). The PDX core facility is equipped with a Biosafety Level 2 (BSL2) surgical suit, laminar flow hood, isoflurane system, CO2 euthanasia machine, CO2 incubators, 1 rack (Allentown) of individually ventilated, microisolator caging system to hold up to 350 animals, microdissection microscope, post-surgery recovery isolators, and surgical and molecular biological equipment. The Transgenic Animal Core Facility (TACF) provides the immunocompromised (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG)) mice for the PDXCF and is situated within a 2,500 sq. ft. specific pathogen-free (SPF) barrier within the animal quarters at the WVU HSC. The laboratory occupies approximately 500 sq. ft. of space within the barrier, and houses the surgical microscopes, CO2 incubators, laminar flow hood, anesthesia machine, molecular biological equipment, and office space for the lab manager. The TACF has a dedicated animal holding room for housing the NSG breeding colony within the barrier, which is separate from the transgenic production colony. The rest of the barrier facility has the capability of housing both mice and rats in individually ventilated, microisolator cages in separate rooms. All cages, bedding and food are autoclaved into the facility, and strict standard operating procedures are in place to ensure protection for the animals housed within the barrier. The NSG animals are offered for sale at a significant discount as compared to commercial vendors and provides WVU investigators the needed animals for cancer cell imaging studies and expansion of human tumor xenografts.