Fee Structure

PDX production is both labor- and time-intensive. The calculated costs include the cost of production of NSG mice, tissue procurement, labor, surgical supplies and equipment and surgical suite usage fees, animal holding per diem, and tumor imaging. Based on these calculations, the production of one animal with successful engraftment of PDX (which averages 50%, with some being more efficient than others) ranges from $1,000-$1,500/per mouse. Propagation of established PDX/per mouse will cost $100-400/mouse depending on growth time (ranges from 30 to 365 days). Table 2 lists the costs of generating and propagating P0 and P1 PDX mice, and the prices charged to WVU investigators. The goal for the PDXCF is to generate ~25-35 new, well characterized PDXs/year. PDXs developed from tissues obtained from WVU Hospitals patients may be commercialized to further support the core.

The Tissue Bank currently charges $500/tumor to recover costs associated with maintaining IRBs, consenting patients, obtaining, analyzing, and releasing samples, and abstracting/de-identifying of medical records.  These costs are ~$100/sample for tumors obtained via the CHTN.  Tissues obtained by individual PIs with their own IRBs can be obtained ‘without charge’ but the associated costs of obtaining IRBs and arranging acquisition of samples are borne by the PI.  Charges will be made monthly during the process of PDX development and will be tracked in CORES, the ordering and tracking system used by the core facilities at WVU.

Services

Non-TME CoBRE Investigators ($)

Generation of P0* PDX (based on 50% success rate)

1,000-1,500

Generation of P1 PDX

150

Cryostorage of PDX (10 vials)

10/month

PDX-derived cells (1 vial 106)

200

PDX frozen tissue (1 vial/3 fragments) ready for grafting/cell isolation

250

PDX paraffin embedded tissue sections (2 per slide)

5